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绿色荧光蛋白

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绿色荧光蛋白 (Green fluorescent protein) 維多利亞多管水母綠色熒光蛋白的結構。 [1] 鉴定 标志 Reginal Pfam(蛋白家族查询站) PF01353 Pfam宗系 CL0069 InterPro(蛋白数据整合站) IPR011584 SCOP(蛋白结构分类数据站) 1ema 现有可用的蛋白结构: Pfam structures PDB(蛋白质数据库) RCSB PDB; PDBe; PDBj PDBsum(蛋白质三维结构站) structure summary 绿色荧光蛋白 (GFP)帶狀圖,來自 PDB 1EMA。 綠色熒光蛋白為基礎的水母鋼鐵雕塑(2006年)陳列在聖胡安島星期五港實驗室(華盛頓,美國),GFP的發現的地方。 綠色螢光蛋白 ( Green fluorescent protein ,簡稱 GFP ),是一个由约238个氨基酸组成的蛋白質,從藍光到紫外线都能使其激發,發出綠色螢光。 [2] [3] 虽然许多其他海洋生物也有类似的绿色荧光蛋白,但傳統上,绿色荧光蛋白(GFP)指首先从維多利亞多管發光水母中分离的蛋白质。這種蛋白質最早是由下村脩等人在1962年在維多利亞多管發光水母中發現。這個發光的過程中還需要冷光蛋白質水母素的幫助,且這個冷光蛋白質與鈣離子(Ca 2+ )可產生交互作用。 在維多利亞多管發光水母中發現的野生型綠色螢光蛋白,395nm和475nm分別是最大和次大的激发波长,它的发射波長的峰點是在509nm,在可見光譜中處於綠光偏藍的位置。绿色荧光蛋白的荧光 量子产率 ( 英语 : Quantum yield ) (QY)为0.79。而從 海腎 ( 英语 : Sea pansy ) (sea pansy)所得的綠色螢光蛋白,僅在498nm有一個較高的激發峰點。 在細胞生物學與分子生物學中,綠色螢光蛋白(GFP)基因常用做報導基因(reporter gene)。 [4] ,綠色螢光蛋白基因也可以轉殖到脊椎動物(例如:兔子)上進行表現,並拿來映證某種假設的實驗方法。通過基因工程,綠色螢光蛋白(GFP)基因能穩轉進不同物種的...

Christian Conference of Asia

The Christian Conference of Asia is a regional ecumenical organisation representing 15 National Councils and over 100 denominations (churches) in New Zealand, Australia, Bangladesh, Burma, Cambodia, East Timor, Hong Kong, India, Indonesia, Laos, Japan, Korea, Malaysia, Pakistan, Philippines, Sri Lanka, Taiwan and Thailand. These councils and churches are committed to working together in mission, leadership development, ecumenical relationships, and issues of social justice. Contents 1 History 2 Member Churches 3 Member Councils 4 Associated bodies 5 References History Representatives of churches, national council of churches, and Christian councils decided to constitute the East Asian Christian Conference during a meeting at Prapat, Indonesia in 1957. It was inaugurated at an assembly in Kuala Lumpur, Malaysia, in 1957 under the theme Witnessing Together. The fifth Assembly in 1973, meeting in Singapore, decided to change the name to Christian Co...

Dalit

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Marginalized communities in the south Asian caste system For the legal term, see Scheduled Castes and Scheduled Tribes. Dalit , meaning "broken/scattered" in Sanskrit and Hindi, is a term mostly used for the ethnic groups in India that have been kept depressed by subjecting them to untouchability (often termed backward castes). [1] Dalits were excluded from the four-fold varna system of Hinduism and were seen as forming a fifth varna, also known by the name of Panchama . Dalits now profess various religious beliefs, including Hinduism, Buddhism, Sikhism and Christianity. As per the latest census, they comprise 16% of India's population (200 million people). [2] The term dalits was in use as a translation for the British Raj census classification of Depressed Classes prior to 1935. It was popularised by the economist and reformer B. R. Ambedkar (1891–1956), who included all depressed people irrespective of their caste into the definition of dalits. [3] Hence t...